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RULE §127.780Biotechnology II (One Credit), Adopted 2021

    (B) analyze data by identifying significant statistical features, patterns, sources of error, and limitations;

    (C) use mathematical calculations to assess quantitative relationships in data; and

    (D) evaluate experimental and engineering designs.

  (4) The student develops evidence-based explanations and communicates findings, conclusions, and proposed solutions. The student is expected to:

    (A) develop explanations and propose solutions supported by data and models and consistent with scientific ideas, principles, and theories;

    (B) communicate explanations and solutions individually and collaboratively in a variety of settings and formats; and

    (C) engage respectfully in scientific argumentation using applied scientific explanations and empirical evidence.

  (5) The student knows the contributions of scientists and recognizes the importance of scientific research and innovation on society. The student is expected to:

    (A) analyze, evaluate, and critique scientific explanations and solutions by using empirical evidence, logical reasoning, and experimental and observational testing so as to encourage critical thinking by the student;

    (B) relate the impact of past and current research on scientific thought and society, including research methodology, cost-benefit analysis, and contributions of diverse scientists and engineers as related to the content; and

    (C) research and explore resources such as museums, libraries, professional organizations, private companies, online platforms, and mentors employed in a STEM field.

  (6) The student prepares for an entry-level career in biotechnology. The student is expected to:

    (A) research and identify career opportunities in genetics, bioinformatics, and fields such as molecular, forensic, medical, regulatory, and agricultural biotechnology;

    (B) identify the significance of recent advances in molecular, forensic, medical, regulatory, and agricultural biotechnology;

    (C) discuss current bioethical issues related to the field of biotechnology;

    (D) create a job-specific resume; and

    (E) develop a career plan.

  (7) The student analyzes academic and professional journals and technical reports. The student is expected to:

    (A) identify the scientific methodology used by a researcher;

    (B) examine a prescribed research design and identify dependent and independent variables;

    (C) evaluate a prescribed protocol to determine the purpose for each of the procedures performed; and

    (D) interpret data and evaluate conclusions.

  (8) The student explores assay design in the field of biotechnology. The student is expected to:

    (A) define assay requirements and optimizations;

    (B) perform statistical analysis on assay design and experimental data such as linearity, system sustainability, limit of detection, and R2 values;

    (C) determine an unknown protein concentration using a standard curve and technique such as a Bradford assay; and

    (D) evaluate enzyme kinetics using a colorimetric assay.

  (9) The student explores applications related to protein expression in the field of biotechnology. The student is expected to:

    (A) describe the fundamental steps in recombinant deoxyribonucleic acid (DNA) technology;

    (B) produce a recombinant protein such as green fluorescent protein (GFP);

    (C) analyze proteins using techniques such as enzyme-linked immunosorbent assay (ELISA), spectrophotometry, and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); and

    (D) isolate a specific protein from a biological sample using techniques such as chromatography and Western blot analysis.

  (10) The student explores applications of recombinant DNA technology and genetic engineering. The student is expected to:

    (A) prepare and maintain tissue cultures commonly used in genetic modification procedures;

    (B) evaluate the effects of changes to growing conditions such as pH, temperature, and growth media;

    (C) evaluate the results of a bacterial transformation using a restriction enzyme digest and Southern blot analysis;

    (D) compare and contrast vectors commonly used in biotechnology applications, including plasmids, adenoviruses, retroviruses, and bacteriophages;

    (E) explain the steps and components of the polymerase chain reaction (PCR); and

    (F) explain applications of CRISPR/Cas9 technology in gene editing and diagnostics.

  (11) The student prepares solutions and reagents for the biotechnology laboratory. The student is expected to:

    (A) demonstrate aseptic techniques for establishing and maintaining a sterile work area;

    (B) prepare, dispense, and monitor physical properties of stock reagents, buffers, media, and solutions;

    (C) calculate and prepare a dilution series;

    (D) determine acceptability and optimum conditions of reagents for experimentation; and

    (E) prepare multi-component solutions of given molarity or concentration and volume.

  (12) The student investigates the role of quality in the biotechnology industry, The student is expected to:

    (A) describe the product pipeline in the biotechnology industry;

    (B) describe the importance of quality assurance and quality control;

    (C) explain the importance of documentation to quality assurance and quality control;

    (D) describe the importance of corrective and preventive action (CAPA);

    (E) describe Quality Management Systems (QMS) components, including inspection, audit, surveillance, and prevention;

    (F) describe Good Manufacturing Practices (GMP), Good Clinical Practices (GCP), Good Documentation Practices (GDP), Good Lab Practices (GLP), and International Organization for Standardization (ISO);

    (G) perform validation testing on laboratory reagents and equipment;

    (H) analyze data and perform calculations and statistical analysis on results of quality-control samples such as standard deviation and percent error; and

    (I) apply and create industry protocols such as laboratory method protocols, standard operating procedures (SOPs), and validation forms.

Source Note: The provisions of this §127.780 adopted to be effective April 26, 2022, 47 TexReg 2166

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